Genome-wide association analysis of seedling root development in maize (Zea mays L.)

Background Plants rely on the root system for anchorage to the ground and the acquisition and absorption of nutrients critical to sustaining productivity. A genome wide association analysis enables one to analyze allelic diversity of complex traits and identify superior alleles. 384 inbred lines from the Ames panel were genotyped with 681,257 single nucleotide polymorphism markers using Genotyping-by-Sequencing technology and 22 seedling root architecture traits were phenotyped. Results Utilizing both a general linear model and mixed linear model, a GWAS study was conducted identifying 268 marker trait associations (p ≤ 5.3×10-7). Analysis of significant SNP markers for multiple traits showed that several were located within gene models with some SNP markers localized within regions of previously identified root quantitative trait loci. Gene model GRMZM2G153722 located on chromosome 4 contained nine significant markers. This predicted gene is expressed in roots and shoots. Conclusion This study identifies putatively associated SNP markers associated with root traits at the seedling stage. Some SNPs were located within or near (\u3c1 kb) gene models. These gene models identify possible candidate genes involved in root development at the seedling stage. These and respective linked or functional markers could be targets for breeders for marker assisted selection of seedling root traits

Not Just a Party in the Parking Lot: An Exploratory Investigation of the Motives Underlying the Ritual Commitment of Football Tailgaters

This study examines the underlying motives within the ritual of football tailgating and the influence of these motives on ritual commitment. Employing an ethnographic approach, methods include participant observation, informal conversations, and formal interviews. Findings indicate that four basic motivations and the dual nature of these motives perpetuate the tailgating ritual: involvement (preparation and participation), social interaction (camaraderie and competition), inter-temporal sentiment (retrospection and prospection), and identity (collectivism and individualism). The data illustrate that the duality of these motives perpetuates consumers’ commitment to the ritual of tailgating and thus motivates participants to continue tailgating over time. Theoretical and sport marketing implications are discussed

Supporting Employee Lactation: Do U.S. Workplace Lactation Benefit Mandates Align with Evidence-Based Practice?

Within the United States, there are governmental benefits and policies in place to support breastfeeding mothers as they return to work. However, the effectiveness and inclusiveness of these policies is not always clear. Because of this, breastfeeding at work, in general, and governmental workplace mandates, specifically, often receive negative press and social media attention as women struggle to reconcile their workplace and lactation demands. To provide evidence-based recommendations for how to best support breastfeeding employees, we use an organizational science perspective to review the existing research for evidence on the (1) effectiveness of the existing legal benefits and supports within the US, and (2) barriers or facilitators that impact benefit success. Through this, we identify areas for improvement and provide suggestions for policy

Effect of infra-red power level on the sintering behaviour in the high speed sintering process

Purpose: To investigate the effects of the infra-red power level on sintering behaviour in the High Speed Sintering process. Design/methodology/approach: Single-layer parts were produced using the High Speed Sintering process, in order to determine the effect of the infra-red power level on the maximum achievable layer thickness, and the degree of sintering. The parts were examined using both optical microscopy and contact methods. Findings: Whilst it was expected that an increase in the infra-red lamp powder might allow an increase in the depth of sintering that could be achieved, as a result of increased thermal transfer through the powder, results in fact indicated that there is a maximum layer thickness that can be achieved, as a result of part shrinkage in the z direction. Optical microscopy images have shown that a greater degree of sintering occurs at higher power levels, which would be expected to correspond to an improvement in the mechanical properties of the parts produced. These images also indicate that the RAM forms in small ‘islands’ on the powder bed surface. However, these islands begin to merge as sintering progresses, to a greater degree as the infra-red lamp power is increased. Research limitations/implications: These results are based only on single layer parts. Further work will examine the sintering characteristics of multiple layer parts. Practical implications: Results have shown that, whilst it is not possible to increase the achievable layer thickness of the parts produced by modifying the infra-red lamp power, the degree of sintering can be improved greatly by increasing the power. Originality/value: High Speed Sintering is an entirely new process which is currently still under development; the results presented here will directly impact the direction of further development and research into this process

Simulation as a Disruptive Innovation in Advanced Practice Nursing Programs: A Report from a Qualitative Examination

Simulation as a pedagogy is used extensively to educate healthcare professionals in both academic and clinical arenas with the intent to improve the delivery of care and patient outcomes. Advanced practice nursing (APN) programs use simulation as a pedagogy even though APN accreditation and certification organizations prohibit substituting simulation hours for the minimum 500 clinical hours. The purpose of this qualitative study was to explore faculty perceptions of educating APN students using simulation. Focus groups were conducted with a convenience sample of APN simulation faculty. Disruptive innovation theory was used by the researchers to guide the data analysis. Themes emerging during analysis included: 1) extrinsic tension and pressure in the midst of chaos, 2) internal vulnerability, and 3) passion and tenacity to remain resilient. The study results provide clarity to understand integration of APN simulation in the current environment, and introduce the impact of simulation as a disruptive innovation